The eggs are then fertilized with the recipient’s husband’s sperms in the laboratory, either by IVF or ICSI and kept in the incubator for two days. Incase the recipient’s husband’s sperm is of poor quality; the eggs can be fertilized by the technique of Intra Cytoplasmic Sperm Injection (ICSI). Two days later, a four-celled embryo is formed. Three days later, a eight-celled embryo is formed or five days later a multi-celled Blastocyst is formed. This embryo (small baby) is then transferred back to the womb either at the four cell, eight cell or the Blastocyst stage. 30 to 40% of such women will become pregnant. Incase they fail to become pregnant they can have a repeat egg donation cycle. Many women undergo two to four cycles and achieve their goal of a child.
Till now, such women could only have a baby through the wonderful route of adoption. However, in the last ten years, a new technique of egg donation has come as a blessing to many such women. B.In India, preference is given to younger couples to adopt children. Couples over the age of 45 can adopt, but find it difficult to do so, because of Governmental preference for younger parents. Such couples can tremendously benefit by egg donation. C.Egg donation can also be perfomed on women who have had multiple cycles of test-tube baby (IVF or ICSI) and have still failed to conceive and become pregnant. D.Besides elderly or menopausal women, egg donation can be done in younger women whose ovaries have prematurely failed or in young women who have undergone radiation or chemotherapy for cancer. Radiation or chemotherapy destroys the eggs and hence these women have a failure of their ovaries. E. Egg donation is also used in patients who are carrying major chromosomal defects so that they do not pass the genetic defect to their children. F. Patients suffering from severe Tuberculosis and severe Endometriosis may also produce poor quality eggs and hence can be treated by egg donation.
This is called testicular sperm extraction (TESE) or micro-TESE, if the surgery is carried out with a microscope. TESE is sometimes carried out before the treatment cycle begins, and under local anaesthetic. The retrieved sperm are frozen. Any discomfort felt by your partner should be mild and can be treated with painkillers. After giving you a local anaesthetic, the doctor will remove your eggs using a fine, hollow needle. An ultrasound helps the doctor to locate the eggs. The embryologist then isolates individual sperm in the lab and injects them into your individual eggs. Two days later the fertilised eggs become balls of cells called embryos. The procedure then follows the same steps as in IVF.
The doctor transplants one or two embryos into your uterus and through your cervix using a thin catheter. If you are under 40 you can have one or two embryos transferred. If you are 40 or over you can have a maximum of three embryos transferred if using your own eggs, or two if you’re using donor eggs. Extra embryos, if there are any, may be frozen in case this cycle isn’t successful. Embryos may be transferred two to three days after fertilisation, or five days after fertilisation. Five days after fertilisation the embryo will be at the blastocyst stage. If you’re just having one embryo transferred (called elective single embryo transfer, or eSET), having a blastocyst transfer can improve your chances of a successful, healthy, single baby.
Pregnancy can be confirmed using blood tests about 13 days after egg aspiration. Pregnancy can be confirmed by ultrasound 30 to 40 days after aspiration.
Not ordinarily. The surgeon must be able to see the follicles in order to guide the needle to the proper spot for retrieval of the eggs whether by sonographic (ultrasound) or surgical methods.
Sperm that can’t be ejaculated but can be collected from the testicles or from the duct where sperm is stored (epididymis). This may be needed if your partner has had an irreversible vasectomy or injury. Problems with getting an erection and ejaculating, due to spinal cord injuries or diabetes, for example. If you have tried IVF you may move on to ICSI if not enough eggs could be retrieved, or if eggs retrieved for IVF were not successfully fertilised.